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Antibody(kinase, phosphatase)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(kinase, phosphatase)

【A】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Akt(Ser473)2011-10-17 WBIF IHCIP
Cell SignalingX500
#9271
rabbit
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
Akt2011-10-17 WBIF IHCIP
Cell SignalingX500
#9272
rabbit
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
AuroraB2011-09-27 EP1009YWBIF IHCIP
EPT100
1788-1
rabbit
m
TCA, 4%PFA, 1%PFA, MeOH
HeLa
calf alkaline phosphatase2011-08-10 WBIF IHCIP
Rocklandx100
2001135
3%PFA (in PBS)
goat

マウス小腸
human alkaline phosphatase2011-08-10 WBIF IHCIP
Rocklandx100
200401142
3%PFA (in PBS)
rabbit

マウス小腸
calf alkaline phosphatase2011-08-10 WBIF IHCIP
Rocklandx100
2004135
3%PFA (in PBS)
rabbit
マウス小腸ではこれがベスト
Best Ab of all alkaline phosphatase Abs for frozen section of mouse small intestine.
マウス小腸
pAkt(Ser473)2011-10-04 D9EWBIF IHCIP
Cell SignalingX2000X200
4060
rabbit
【4%PFA+0.2%Saponin】:○
Mono

HL-60細胞
Akt2011-10-04 WBIF IHCIP
Cell SignalingX1000
9272
rabbit
Poly

HL-60細胞

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Calcineurin/PP2B B2011-08-14 WBIF IHCIP
upstate
07-069
rabbit
p

rat b-CaM Kinase II2011-08-10 WBIF IHCIP
Zymed
139800
mouse
m

human calcineurin/PP2B B2011-08-10 WBIF IHCIP
Upstate
7069
rabbit
p

human calcineurin/PP2B B2011-08-10 WBIF IHCIP
Upstatex1000
7069
rabbit
p

マウス脳
Calcineurin (α-Subunit)2011-08-10 a-subunit CN-A1WBIF IHCIP
Sigma
C1956
mouse
m

Calcineurin (α-Subunit)2011-08-10 a CN-A1WBIF IHCIP
Sigma
C1956
mouse
m

CaMK IV2011-08-14 WBIF IHCIP
BD transduction
C28420
mouse
m

CaMK II2011-08-14 WBIF IHCIP
BD transduction
C89220
mouse
m

Cdk5 Ab-42011-08-10 DC17+DV34WBIF IHCIP
NeoMarkers
MS-1059-p
mouse
m

試してない?
Not used
Cdc2 p34 (C-19)2011-10-17 WBIF IHCIP
Santa CruzX1000
sc-954
rabbit
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
human calreticulin2011-08-10 WBIF IHCIP
Stressgenx100
SPA-600
3%PFA (in PBS)
rabbit
p

マウス精巣、膵臓

【D】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho DARPP-322011-08-14 Thr34WBIF IHCIP
Cell Signaling
2304S
rabbit
p

2本ある

【F】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
FAK2011-09-27 WBIF IHCIP
Santa Cruz100
sc-1688
mouse
m
4%PFA 大変弱い
Very weak staining
HeLa

【I】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
IRAK2011-08-29 7WBIF IHCIP
BD Transductionx500x500
mouse
Mono, IgG1

Hela細胞

【K】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
KAP2011-08-14 WBIF IHCIP
BD transductionx1000x100
610334
3%PFA (in PBS)
mouse
m

マウス神経細胞

【L】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
LAR2011-08-14 WBIF IHCIP
BD transduction
L33420
mouse
m

【M】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
MYPT1 pT850 (chick) / pT853 (human)2013-02-01 WBIF IHCIP
Upstate (Millipore)X1000
36-003
rabbit (poly)
cell lysate調製時に急速に脱リン酸化されるため、TCA処理またはSDS sample bufferによる調製を推奨 Samples should be directly prepared by TCA or SDS sample buffer because of the rapid dephosphorylation in preparation.
MKP22011-08-14 WBIF IHCIP
BD transductionx1000
610850
mouse
m

マウス神経細胞
Phospho-p44/42 MAP kinase (pT202/pY204)2011-09-27 WBIF IHCIP
Cell Signaling100
9106S
mouse
monoclonal
4%PFA
MDCK II
Myosin phospatase2011-09-27 WBIF IHCIP
Covance100
PRB-457C
rabbit
p
1%PFA>TCA
HeLa

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PhosphoTyrosine2011-08-29 4G10WBIF IHCIP
Upstatex1000
mouse
Mono

Hela細胞
PKCm2011-08-29 C-20(sc-639)WBIF IHCIP
Santa Cruzx500
rabbit
Poly

Hela細胞
PKARIIbeta2011-08-29 45WBIF IHCIP
BD Transduction Laboratoriesx10000
mouse
Mono, IgG1

Hela細胞
PKARIIalpha2011-08-29 40WBIF IHCIP
BD Transduction Laboratoriesx10000
mouse
Mono, IgG1

Hela細胞
PAR-1b/MARK2/EMK 2011-10-14 WBIF IHCIP

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PTP10D (drosophila)2016-08-10 8B22F5WBIF IHCIP
Developmental Studies Hybridoma Bank (DSHB)x100
4%PFA (in phosphate buffer)
Mouse
0.1% TritonX-100/PBS
IgG1

Drosohila imaginal disc

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PAR-1a/MARK3/c-TAK1 2011-10-14 183.A10.A3WBIF IHCIP
Millipore/Upstate x 300
05-680
2% PFA 0.5%Triton, RT 15min
mouse
Mono, unknown
【抗原の種、部位】human full length
MDCK cells
PAK2(C17A10)2011-10-17 WBIF IHCIP
Cell SignalingX1000
#2615
rabbit
Mono, IgG
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
Plk42011-10-17 WBIF IHCIP
Cell SignilingX1000X100
#3258
rabbit
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
p70S6 Kinase2011-09-27 WBIF IHCIP
Cell Signaling1:1000
#9202
rabbit
p

phospho p70S6 Kinase (T389)2011-09-27 WBIF IHCIP
Cell Signaling1:1000
#9205
rabbit
p

rat PKC g2011-08-10 WBIF IHCIP
Zymedx100
133800
3%PFA (in PBS)
mouse

マウス小脳

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKD 12014-01-07 WBIF IHCIP
Cell Signaling Technologyx500x100
2052
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
human PP1 alpha2011-08-10 WBIF IHCIP
Biocomparex1000
2581S
rabbit
p

マウス脳
Plk12011-10-17 35-206WBIF IHCIP
invitrogenX500
37-7000
mouse
Mono, IgG1-kappa
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
pPAK(Thr423)2011-10-04 WBIF IHCIP
InvitrogenX1000
44-942G
rabbit
Poly

HL-60細胞

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKD 32014-01-07 WBIF IHCIP
Cell Signaling Technologyx1000
5655
rabbit
IgG

WB: several tissues

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
aPKC iota2011-10-14 23/PKCiWBIF IHCIP
BD Bioscience x 1000x 100
610176
2% PFA 0.5%Triton, RT 15min
mouse
Mono、IgG2b
【抗原の種、部位】human a.a. 404-587
aPKC iota はllamdaと同じである
APKC iota is the same as aPKC lamda
MDCK cells
PP12011-08-10 protein phosphatase 1WBIF IHCIP
BD Transductionx1000
610373
mouse
m

マウス脳
PP2A2011-08-14 WBIF IHCIP
BD transductionx1000
610555
mouse
m

マウス脳
human PP1 delta2011-08-10 WBIF IHCIP
Upstatex1000
7270
rabbit

マウス脳
phospho p42/442011-09-22 WBIF IHCIP
Cell Signaling Tech1/50-1/100
9101
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
poly
incubate w/ Phosphatase inhibitors
mouse E6.5 embryo.MEF
phospho-PKA substrate2011-08-14 WBIF IHCIP
Cell Signaling
9621S
rabbit
p

human PKD22011-08-10 BL844WBIF IHCIP
Bethyl
A300074A
rabbit

PKD22011-08-14
About Product
MonoclonalWBIF IHCIP
Abcam
ab51250
rabbit
IgG

human PKC mu2011-08-10
About Product
PolyclonalWBIF IHCIP
Abcam
ab5284
rabbit
IgG

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKD 12014-01-07 WBIF IHCIP
Sigmax100000x100
P 3987
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKA RIα2011-08-14 WBIF IHCIP
BD transduction
P19920
mouse
m

Protein Kinase D2011-08-10 WBIF IHCIP
SIGMA
P3987
rabbit

PKA RIα2011-08-14 WBIF IHCIP
BD transduction
P53620
mouse
m

PKA RIIβ2011-08-14 WBIF IHCIP
BD transductionx1000x100
P54720
3%PFA (in PBS)
mouse
m

マウス神経細胞
aPKC (zeta and lambda)2011-10-14 WBIF IHCIP
Santa Cruz x 1000x 300
sc-216(C-20)
2% PFA 0.5%Triton, RT 15min
rabbit
Poly
【抗原の種、部位】rat C-terminus
MDCK cells

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKD 12014-01-07 WBIF IHCIP
St. Cruz Biotechnology C-20x100000x100
SC-639
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung
PKD 12014-01-07 WBIF IHCIP
St. Cruz Biotechnology D-20x2000x100
SC-935
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
rabbitαPKCμ2011-08-10 C-20WBIF IHCIP
Santa Cruz
sc639
rabbit
p

Phosphorylated-Tyrosine(p-Tyr)2011-11-26 PY-99WBIF IHCIP
Santa Cruzx50
sc7020
4%PFA +0.1%Triton
mouse IgG2b
IgG2b

Drosophila embryo, discs, nervous system
PAK12011-10-04 WBIF IHCIP
Santa CruzX1000
SC882
rabbit
Poly

HL-60細胞
human PKA b cat2011-08-10 C-20WBIF IHCIP
Santa Cruz
sc904
rabbit

human PKC m2011-08-10 D-20WBIF IHCIP
Santa Cruz
sc935
rabbit
p

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ROCK12013-02-01 EPR638YWBIF IHCIP
Epitomicsx500x200
1953-1
4% PFA
rabbit (mono)

HeLa

【r】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
RIPK3 (mouse)2016-05-16 WBIF IHCIP
Prosci1/1000
2283
Rabbit

MEF
RIPK1 (human/mouse)2016-08-10 WBIF IHCIP
BD Biosciences1/500
610459
Mouse
IgG2a

MEF, HT29

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Src2011-09-27 WBIF IHCIP
Santa Cruz100
sc-8056
mouse
m
Focal adhesion染まらず。おそらく使用不可
Not stain focal adhesion.
HeLa

【U】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ULK12011-09-27 H-240WBIF IHCIP
Santa Cruz1:1000
sc-33182
rabbit
p

【Z】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Zip Kinase2011-09-27 WBIF IHCIP
Sigma100
Z0134
rabbit
p
1%PFA on ice
MDCK II